The Impact of Septum Swelling on Sample Volume and Injection Precision

3.1 Injection Systems. The injector port is a hot chamber containing a glass liner where the compounds are volatilized and swept by a stream of inert carrier gas into the column. This chamber is closed by a rubber or microseal septum which is perforated by the needle of a syringe to introduce the sample.

sample on needle), deactivated, 870 L volume. Glass nub ensures that gap remains below liner for split injection. Efficient, for most applications, including active compounds. Fail-safe insertion into injection port. Needle length is important. 18740-80190 Liner with Jennings cup, no glass wool, 800 L volume. Use

The guidelines for sample injection volumes are as follows. If the injection volume is too large, the peak shape will become deformed, or the injection port will become dirty, leading to problems. Liquid samples: Approx. 1 to 2 µL Gas samples: Approx. 0.2 to 1 mL. Liquid samples become a gas at the stage of injection into the column.

Septum in injection port is too thick or too tight • Loosen the nut slightly to allow for improved injection Septum coring • Use a septum-less injector system (i.e. Merlin Microseal) – Fibers: • p/n 5182-3445: Merlin Microseal 100 psi nut • p/n 5182-3444: Merlin Microseal general-purpose replacement Microseal 30 SPME troubleshooting

The autosampler component of a HPLC system is used to increase sample throughput, improve the precision of injections, and allow for unattended operation. Its main components are a needle or syringe, a metering device to measure sample volume, and a sample loop. There is also a valve used to control the hydraulic path of the eluent.

The guidelines for sample injection volumes are as follows. If the injection volume is too large, the peak shape will become deformed, or the injection port will become dirty, leading to problems. Liquid samples: Approx. 1 to 2 µL Gas samples: Approx. 0.2 to 1 mL. Liquid samples become a gas at the stage of injection into the column.

4. Sample Injection. 4.1. Sample Injection Volume. The guidelines for sample injection volumes are as follows. If the injection volume is too large, the peak shape will become deformed, or the injection port will become dirty, leading to problems. Liquid samples: Approx. 1 to 2 µL Gas samples: Approx. 0.2 to 1 mL.

Why Inject Water? •Convenient • Aqueous samples (waste water, drinking water, etc.) • Biological samples •Necessary • Purge & Trap/Static Headspace Potential Problems with Water •Injector Issues • Large expansion volume • Backflash Potential Problems with Water •Detector Issues • Extinguish FID flame • Decrease sensitivity of ECD • “Wet” MSD

n The sample manager injects up to 75% of sample loop volume with good linearity. The minimum for 1-µL, 100-µL, and 250-µL loops is 20%, and the minimum for 2-µL through 50-µL loops is 10%. n Air gaps bracket the “pre-sample + sample + post-sample” volume. n The sample needle aspirates the specified injection volume plus

The Arc HPLC System uses a flow-through needle injector design that delivers high injection precision across the range of analytical injection volumes, irrespective of the sample diluent. To demonstrate injection precision and linearity, caffeine standard and acenaphthene standard were injected repeatedly onto an analytical column (4.6 x 50 mm

The measurement of MRI DWI volume within 6 hours is useful, and volumes (≥80 mL) predict rapid fulminant course (Class I; Level of Evidence B). A noncontrast CT scan of the brain is a useful first-line diagnostic test and modality of choice to monitor patients with hemispheric cerebral or cerebellar infarcts with swelling.

Mar 1, 2012 · It is also possible to set up a short injection time that causes partial sample loop transfer, but this may impact results repeatability in an unfavourable way. Valve V2 can be left open during the sampling interval to vent the remaining headspace gases from the vial, or it can be closed when the six-port valve is actuated.

Increase Injection Volume • liner dependent (use the Pressure-Volume Calculator) • 2 uL maximum Reduce Split Ratio • go from 50:1 to 10:1 • 10:1 practical lower limit for liquid injections (for 250 - 320 um i.d. columns) • 1:1 possible for gas injections with correct liner • Keep TOTAL INLET FLOW at 20 mL/min or higher

for linearity (R2) across this injection volume range exceeds 0.99999, demonstrating the true flexibility of the injection design. The repeatability of six replication injections across this same range was below 0.2% for all components and typically below 0.1% (Figure 2). In addition to injection precision and linearity, accuracy

The Merlin MicroSeal injection port septa is the perfect substitute to use in split/split less injection and SPME. It has the capacity to hold more than 2000 syringes depending upon the type of the syringe. Instrument downtime for septa changes and port liner changes is greatly minimized. It offers. Compare this item.