Jan 08, 2021 · The Aijiren II HPLC instrument described above was used with a stainless-steel column packed with ReproSil-XR 120 C18, 5 μm, 150 mm x 4.6 mm (Dr. Maisch GmbH, Ammerbuch, Germany) and a guard column containing the same material. The column oven was kept at 35.0°C.