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Determination of ethanol in blood using headspace gas The sample injection volume was 0.2 mL. The samples were prepared in headspace vials using a water bath at a temperature of 50 °C for 10 min. 2.3. Methods 2.3.1. Preparation of samples, internal standards, calibrators, controls and validation parameters Initially, the blood sample was prepared by diluting 1: 5 with deionised water and then The ChromaTography and Sample preparaTion Terminology guide addition, with new sample prepara-tion...

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